Direct quantitation of platelet-associated IgG by electroimmunoassay

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Direct quantitation of platelet-associated IgG by electroimmunoassay.

An electroimmunoassay was applied to the quantitation of platelet-associated IgG (PAIgG). Protein solubilized by Triton X100 from washed platelets was electrophoresed at pH 5.0 in agarose gels containing carbamoylated rabbit anti-human IgG (pI approximately equal to 5.0). Because the rabbit antibody is immobilized under these conditions, while PAIgG is freely mobile, rocket precipitates were pr...

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Quantitation of platelet-associated IgG by radial immunodiffusion.

Platelet-associated IgG (PAIgG) was measured by a simple radial immunodiffusion technique using washed solubilized platelets and commercially available immunoplates. Subjects with normal platelet counts had PAIgG levels of 1.5--7.0 fg/platelet. Subjects with idiopathic immune thrombocytopenic purpura (ITP) had levels ranging from 5.7 to 70.5 fg/platelet. All patients with recurrent ITP and 85% ...

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The quantitation of platelet-associated IgG on cohorts of platelets separated from healthy individuals by buoyant density centrifugation.

Evidence suggests that as platelets age in the circulation they become progressively smaller and less dense through the loss of protein. The smallest, least dense platelets have a significantly shortened survival, but the mechanism of clearance of these platelets is not known. To evaluate whether the binding of IgG could play a role in the clearance of senescent platelets, we measured platelet ...

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Platelet-associated IgG in patients with lymphoma.

Levels of platelet-associated IgG (PA-IgG) were studied in 72 patients with Hodgkin's (HD) and non-Hodgkin's lymphoma (NHL). Thirty-nine percent of patients with HD and 20% of patients with NHL had elevated PA-IgG levels. There was a positive correlation between disease activity and the presence of PA-IgG in HD and NHL. In patients with HD, PA-IgG strongly correlated with extent of disease and ...

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Passive haemagglutination inhibition for quantitation of red cell associated IgG.

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ژورنال

عنوان ژورنال: Blood

سال: 1982

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v60.1.54.54